Marcenaro e, della chiesa m, bellora f, parolini s, millo r, moretta l, moretta a. Gm csf is a protein secreted by macrophages, t cells, mast cells, nk cells, endothelial cells and fibroblasts. Gm csf protein is fully biologically active when compared to standard. The lancet clinical practice human recombinant gmcsf in allogeneic bonemarrow transplantation for leukaemia. Equine gmcsf yeastderived recombinant protein 5 micrograms. Another use of gm csf is as a vaccine adjuvant and it was also claimed to improve the general health condition of aids patients although this is a controversial issue and it was recently found that gm csf actually induces the expression of hiv1in monocytes.
The dttreduced protein migrates at approximately kda and the nonreduced protein migrates at approximately 11kda by sdspage. Cell proliferation was measured after 48 hours of culture using a. In addition, it cooperates with g csf in promoting tumor cell proliferation and invasion. Recombinant human granulocyte macrophage colonystimulating factor gmcsf is a bioactive. Gm csf was first cloned in 1985, and soon afterwards three potential drug products were being made using recombinant dna technology. Gmcsf is a growth factor secreted by several cell types from the innate. These gm csf proteins are produced with different tags, such as his tag, hfc tag. Recombinant human gmcsf carrierfree flow cytometry. Gm csf is involved in many biological responses including the growth and development of granulocyte and macrophage progenitor cells, stimulation and the initiation of differentiation of myeloblasts. Granulocytemacrophage colonystimulating factor, csf 2, mgi1gm, pluripoietin. Granulocytemacrophage colonystimulating factor, csf. Granulocytemacrophage colonystimulating factor gm csf is a protein secreted by macrophages, t cells, mast cells, nk cells, endothelial cells and fibroblasts. The specific activity of recombinant human gmcsf is approximately 1.
The protein migrates as 1828 kda on a sdspage gel under reducing r condition due to glycosylation. Recombinant gmcsf and gcsf proteins have been used to treat various diseases including. Gm csf r alpha fcb chimera separates into a number of isoforms with a pi between 6. The molecular sequence of endogenous human gm csf was first identified in 1985. The ec50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. Molecular cloning and biological activity of the recombinant protein. Recombinant human granulocytemacrophage colonystimulating factor rhugm csf produces doserelated therapeutic and toxic effects. Recombinant human g csf carrier free human g csf was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Granulocyte colonystimulating factor and granulocyte. Human gmcsf r alpha protein, fc tag acrobiosystems. The therapeutic potential of recombinant gmcsf sargramostim has been. Two new recombinant rec ndv strains each containing the human gm csf gene incorporated at different positions within the viral genome. Gibco gmcsf recombinant human protein recombinant human granulocyte macrophage colonystimulating factor gmcsf is a bioactive protein intended for use in cell culture applications manufacturer.
Targeting of il2 and gmcsf immunocytokines to a tumor vaccine. Granulocyte macrophage colony stimulating factor mouse recombinant produced in e. Equine gm csf protein has a molecular weight of 15. Gm csf stimulates stem cells to produce granulocytes neutrophils, eosinophils, and basophils and monocytes. Recombinant human gmcsf carrier free gmcsf plays a key role in signaling emergency hemopoiesis predominantly myelopoiesis in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. Granulocytemacrophage colony stimulating factor gm csf is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. In a randomised, doubleblind trial 20 patients with leukaemia received human recombinant granulocyte macrophage colonystimulating factor gmcsf and 20 received placebo, for 14 days after allogeneic, matched sibling, bonemarrow transplantation. Mouse gm csf recombinant protein is purified gm csf produced in yeast. This molecular mass is due to glycosylation, which is absent when this cytokine is expressed in e. Recombinant human gmcsf carrier free gm csf plays a key role in signaling emergency hemopoiesis predominantly myelopoiesis in response to infection, including the production of. The ec50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The equine gm csf csf 2 endotoxinfree recombinant protein can be used in cell culture, as an gm csf elisa standard, and as a western blot control.
Gm csf protein mouse recombinant csf2 antigen prospec. Human gm csf recombinant protein is purified gm csf produced in yeast. Coli is a single, nonglycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285. Recombinant human gmcsf choexpressed protein 7954gm010. Pdf a recombinant human gcsfgmcsf fusion protein from e. Granulocyte macrophage colonystimulating factor human. Recombinant human granulocyte and granulocytemacrophage. Human gm csf, along with other colony stimulating factors, are key drivers of differentiation and proliferation of hematopoietic precursors. Here the authors show that the inflammatory cytokine, granulocyte.
Gm csf mouse is purified by proprietary chromatographic techniques. Granulocyte colonystimulating factor g csf promotes neutrophil proliferation, differentiation, and survival. Granulocytemacrophage colonystimulating factor wikipedia. Gmcsf protein, human recombinant animal free sigmaaldrich. Gmcsf mouse recombinant protein, shenandoah biotechnology vwr. This hypothesis prompted us to examine the safety and effectiveness of recombinant human granulocytemacrophage colonystimulating factor gm csf for treatment of idiopathic crohns disease. Aortic dissection and intramural haematoma are caused by separation of the aortic wall via an unknown mechanism. Gm csf is also involved in the treatment of crohns disease. Bovine gm csf recombinant protein is purified gm csf produced in yeast. Distribution of granulocyte colonystimulating factor gcsf. Protective effects of recombinant human granulocyte macrophage colony stimulating factor rhugmcsf on h1n1 influenza virus infection was studied in vivo and in vitro.
Among these gm csf proteins, there are 7 human gm csf protein, 3 mouse gm csf protein, 2 rat gm csf protein. Cantonetti m, racanelli v, teofili l, ferrara r, amadori s, castoldi gl, dammacco f, levis a 2000. Animalfree recombinant human g csf is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 21 to 25 kda. Recombinant human gmcsf r alpha protein, cf 706gr050. Macrophage colonystimulating factor from mouse mcsf, recombinant, expressed in e. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. In this randomized, controlled trial, inhaled recombinant human gm csf was. The gmcsf receptor gmcsfr is expressed on neutrophils, dendritic cells, macrophages, eosinophils, and endothelial cells. Glycosylation contributes to stability in cell growth media and other applications. Mice were infected with h1n1 influenza a viruses and rhugmcsf at doses of 0. It acts by binding to specific cellsurface receptors g csf r, which are expressed.
Production of recombinant ndv viruses with the gm csf gene. Gmcsf protein, human recombinant animal free the gmcsf protein was initially characterized as a factor that can support the in vitro colony formation of granulocyte macrophage progenitors. Granulocyte macrophage colonystimulating factor is. Reproducible results in inhibition activity assays.
All these gm csf protein are expressed by different host cells. The ability of recombinant gm csf to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recombinant human gmcsf animal free gm csf plays a key role in signaling emergency hemopoiesis predominantly myelopoiesis in response to infection, including the production of. Molecular mass the 125 amino acid nterminal methionylated recombinant protein has a predicted. The specific activity of recombinant human gm csf is approximately 1. Granulocytemacrophage colonystimulating factor promotes. Recombinant gm csf is a bioactive protein intended for use in cell culture applications.
Granulocytemacrophage colonystimulating factor gm csf, also known as colonystimulating factor 2 csf2, is a monomeric glycoprotein secreted by macrophages, t cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. In a randomised, doubleblind trial 20 patients with leukaemia received human recombinant granulocyte macrophage colonystimulating factor gm csf and 20 received placebo, for 14 days after allogeneic, matched sibling, bonemarrow transplantation. Pdf production of functional recombinant human gmcsf rhgm. Recombinant swine gm csf is intended for use in cell culture applications. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. Two different commercial sources of recombinant human rh gm csf were used in these experiments. A the biological activity of human recombinant gmcsf was tested by its ability to promote the proliferation of tf1 cells. Csf was initially characterized as a factor that can support the in vitro colony formation of granulocyte. Recombinant gmcsf and gcsf proteins have been used to treat various diseases including cancer and hematopoietic diseases and to isolate. Gm csf protein, human recombinant animal free the gm csf protein was initially characterized as a factor that can support the in vitro colony formation of granulocyte macrophage progenitors. Qgrwrlqhyho gm csf is a protein secreted by macrophages, t cells, mast cells, nk cells, endothelial cells and fibroblasts. Gm csf inhibits neutrophil migration and enhances the functional activity of the mature endcells. Recombinant human granulocyte macrophage colonystimulating factor gm csf is a bioactive protein intended for use in cell culture.
Gm csf is involved in many biological responses including the growth and development of granulocyte and macrophage progenitor cells, sti. Protective effects of recombinant human granulocyte. Pdf production of functional recombinant human gmcsf rhgmcsf under specific promoter in. Gm csf stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocytemacrophage colonies from bone marrow cells. Gibco gmcsf recombinant human protein phc2015 print. Gm csf exerts its biological effects through a heterodimeric receptor complex composed of gm csf r alpha cd116 and the signal transducing common beta chain cd1 which is also a component of the highaffinity receptors for il3 and il5 11, 12. G csf differs from gm csf in its specificity of action on developing and mature neutrophils, its effects on neutrophil kinetics, and its toxicity profile. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0. Serious adverse events developed in 6 patients in the gm csf group and in 3 patients in the placebo group. Recombinant human gmcsf carrier free gm csf plays a key role in signaling emergency hemopoiesis predominantly myelopoiesis in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. Granulocyte colonystimulating factor g csf and granulocytemacrophage colonystimulating factor gm csf enhance the antimicrobial functions of mature neutrophils. Recombinant human gmcsf, animalfree protein afl21501m. Granulocyte macrophage colonystimulating factor an overview.
Human gm csf r alpha, fc tag grah5255 is expressed from human 293 cells hek293. Gm csf increases leukocyte and reticulocyte counts and. Description granulocyte macrophage colonystimulating factor gmcsf is a hematopoietic growth factor produced by various cell types such as t and b cells, macrophages, endothelial cells and fibroblasts, in response to cytokine or inflammatory stimuli. Pdf disposition of recombinant human granulocytemacrophage. Gm csf protein human recombinant csf2 antigen prospec. Recombinant human granulocyte macrophage colonystimulating factor gm csf is a bioactive protein intended for use in cell culture applications.
Gm csf signals through a receptor complex composed of gm csf r alpha and the common beta chain beta c with. The ed50 as determined by a cell proliferation assay using human tf1 cells is less than 0. Description granulocyte macrophage colonystimulating factor gm csf. Granulocyte macrophage colony stimulating factor an. The pharmaceutical analogs of naturally occurring gm csf. Recombinant,human,gmcsf,ruo 550068 bd biosciencesus. A recombinant human gcsfgmcsf fusion protein from e. Gmcsf recombinant human protein thermo fisher scientific.
Granulocytemacrophage colonystimulating factor, csf2, mgi1gm, pluripoietin. Recombinant,mouse,gmcsf,ruo 554586 bd biosciencesus. Gm csf promotes immune system development and regulates neutrophil function during infection. A the biological activity of human recombinant gmcsf, acf was tested by its ability to promote the proliferation of tf1 cells. Atsushi mizoguchi, akira andoh, in animal models for the study of human disease, 20. The recombinant animal free human gmcsf is manufactured using all nonanimal reagents. Granulocyte macrophage colonystimulating factor gm csf is a hematopoietic growth factor that contributes in promoting myeloid cell development, maturation, and survival. Gm csf increases leukocyte and reticulocyte counts and also recruits eosinophils, macrophages and neutrophils to sites of inflammation.
A the biological activity of human recombinant gm csf was tested by its ability to promote the proliferation of tf1 cells. In addition, gm csf binds a naturally occurring soluble form of gm csf r. Treatment of active crohns disease with recombinant human. Gm csf plays a key role in signaling emergency hematopoiesis predominantly myelopoiesis in response to infection. The human and murine molecules are speciesspecific and exhibit no crossspecies reactivity. Powles, leukaemia unit, royal marsden hospital, downs road, sutton, surrey sm2 5pt, uk.
Gmcsf is involved in many biological responses including the growth and development of granulocyte and. Authentic glycosylation, maturation, and folding lead to greater stability and activity than cho and e. Emerging applications of recombinant human granulocyte. Granulocytemacrophage colonystimulating factor gm csf is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and t cells. In addition, gm csf binds a naturally occurring soluble form of gm csf r alpha. Molecular mass the 125 amino acid nterminal methionylated recombinant protein has a predicted molecular mass of 14. Recombinant human gmcsf choexpressed protein 7954gm.
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